Introduction: The association of neutrophil-to-lymphocyte ratio (NLR) with muscle mass, strength, and functional capacity was evaluated in hemodialysis (HD) patients. Methods: A cross-sectional study was performed evaluating 68 adults and older adults of both sexes undergoing HD. Bioelectrical impedance analysis (BIA) was used to obtain the appendicular skeletal muscle mass (ASMM). Handgrip strength (HGS) and 4 m-gait speed (GS) tests were performed to assess the muscle strength and functional capacity, respectively. The pollicis muscle thickness (APMT) was used as indicator of muscle mass. Multiple regression analyses were carried out to assess the association of NLR with ASMM, HGS, GS, and APMT, adjusting for confounders (age, sex, HD time, and smoking habit). Results: Out of 68 (59.1 ± 14.2y) HD patients evaluated, most were men (64.7%) and were overweight (BMI = 26.81 ± 4.8 kg/m²). After adjustments for confounders, NLR was not associated with ASMM (β: 0.029, p=0.66), HGS (β: -0.007, p=0.66), GS (β: -0.310, p=0.72) or APMT (β: -0.061, p=0.14). Conclusion: NLR is not associated with muscle mass, strength, and functional capacity in HD patients.