Introduction: The present study evaluated the effect of repeated low-dose and high-dose postbiotics obtained from strains of Lactobacillus gasseri (CCT 7860), Lactobacillus casei (CCT 7859), Lactobacillus paracasei (CCT 7861), Streptococcus thermophilus (ATCC 19258) and Bifidobacterium lactis (CCT 7858) in the inflammatory effects in different tissues and damage in the liver and kidney. Methods: The experimental protocol consisted of fifteen probiotics administrations in the low-dose (10 mg/postbiotics/day) and high-dose (1 g/postbiotics/day) by the intragastric (i.g.) route. On the sixteen day, mice were euthanized and the blood, liver, kidney, brain and gut were removed for different analysis: myeloperoxidase (MPO) activity, nitrite levels and cytokine levels (interleukin (IL)-1β, IL-10, IL-6). Results: The low toxicity of postbiotics is supported by the observation that, after its administration for 15 consecutive days, there were no changes in the levels of aspartate aminotransferase (AST) and alanine transaminase (ALT), creatinine and in the histopathology of the intestine of mice, confirming the safety of its use. Conclusion: Our study shows that postbiotics supplementation is safe and no toxic even in high doses.